The transport layer is a very interesting layer that serves numerous important functions. Its first purpose is to choose the protocol over which the data is to be transmitted. The two most common protocols in the transport layer are TCP (Transmission Control Protocol) and UDP (User Datagram Protocol); with TCP the transmission is connection-based which means that a connection between the computers is established and maintained for the duration of the request. This allows for a reliable transmission, as the connection can be used to ensure that the packets all get to the right place. A TCP connection allows the two computers to remain in constant communication to ensure that the data is sent at an acceptable speed, and that any lost data is re-sent. With UDP, the opposite is true; packets of data are essentially thrown at the receiving computer — if it can’t keep up then that’s its problem (this is why a video transmission over something like Skype can be pixelated if the connection is bad). What this means is that TCP would usually be chosen for situations where accuracy is favoured over speed (e.g. file transfer, or loading a webpage), and UDP would be used in situations where speed is more important (e.g. video streaming).
With a protocol selected, the transport layer then divides the transmission up into bite-sized pieces (over TCP these are called segments, over UDP they’re called datagrams), which makes it easier to transmit the message successfully.